The study was conducted in order to use floral organ, such as stem, petal, ovary, and anther tissue of Lilium lougiflorum, as the materials for bulblet proliferation through the experiment on concentration of plant growth regulators suitable for callus induction and organogenesis, difference of totipotency between flower-bud sizes (stages), and effects of cold treatment on floral organ before inoculation.
The results obtained were summarized as follows. Percentage of bulblet formation and number of bulblet/explant from stem tissue were higher in 0.1ppm NAA and 0.1ppm kinetin, while that of petal, ovary, and anther tissue, in 1.0ppm IAA, or 1.0ppm IAA and 0.1ppm kinetin.
Optimum placing time of stem tissue was the stage which reached at 1.9±0.23×0.8±0.12㎝ and that of ovary, petal and anther tissue, 4.4±0.11 × 1.3±0.09㎝. Percentage of bulblet formation from stem, petal, and ovary tissue was higher in the media with 1.0ppm IAA or 1.0ppm IAA+0.lppm kinetin, but that from anther tissue, in the medium with 1.0ppm IAA, while response to both media in mean fresh weight and no. of bulblets/explant was different among tissues cultured.
Percentage of bulblet formation was not increased by cold treatment except petal and ovary tissue of which flower-bud size reached 1.1±0.02 × 0.6±0.08㎝, but it was affected in increase of number of bulblet/explant and mean fresh weight.
Percentage of bulblet formation and proliferation of bulblet from bulb scales cultured in mid June were higher than in late July and optimum auxin concentration was 0.1ppm NAA. Stem, petal, and ovary tissues were more efficient than bulbscale tissue as cultural materials.
Considering above mentioned results, cultivation time of stem tissue was optimal when flowerbud size was 1.9±0.23 × 0.8±0.12 and that of petal and ovary tissue was 4.4±0.11 × 1.3±0.09㎝ for differentiation and proliferation of bulblet in the medium supplemented with 1.0ppm IAA alone or combined with 0.1ppm kinetin.
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